BIOFIRE FILMARRAY SYSTEM is a FDA-approved multiplex PCR system having 4 different panels: CSF Panel, Blood Culture Panel, Respiratory Panel and G.I. Panel. Blood culture panel (BCID Panel) is for detection of causative organism of sepsis due to bacteria or fungi. The most important feature of the panel is that it can diagnose the drug resistant of bacteria (MRSA, VRSA, VRE, KPC) only in 2 hours. It includes 16 bacteria (Acinetobacter baumanii, Haemophilus influenzae, Neisseria meningitidis, Pseudomonas aeruginosa, Enterobacter cloacae complex, Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, Proteus species, Seratia mersescense, Enterococcus species, Listeria monocytogenes, Staphylococcus aureus, Streptococcus agalactae, Streptococcus pyogenes, Streptococcus pneumoniae) & 5 fungi (Candida albicans, Candida krusei, Candida glabrata, Candida parapsilosis, Candida tropicalis). Sepsis is a life-treated condition unless treated immediately. And detection of the causative pathogen along with drug resistance pattern is of utmost importance to start the treatment on an urgent basis. It will reduce the morbidity and the hospital stay of the patient. 5-10 ml of blood is to be collected in blood culture bottles with aseptic precautions and to be sent to the laboratory at the earliest. Different body fluids (Pleural fluid, Ascitic fluid, Pericardial fluid, Peritoneal fluid, Synovial fluid) can also be processed in this panel to diagnose the deep-seated infections which may be sometimes systemic and life-threatening in critically ill and paediatric patients. BIOFIRE BCID Panel gives the diagnosis of the organism only in 2 hours after flagging in the automated BacT/Alert instrument. It is a gold standard technique for the detection of the pathogen with the sensitivity of 96% and specificity of 99%.