|Samples||All Specimens in Sterile Container except Sputum|
|Report turn around time||Next Day|
Acid fast staining is a standard staining technique for demonstration of acid fast bacilli i.e. Mycobacterium tuberculosis (TB BACILLI) and other atypical Mycobacteria. Ziel-Neelsen Technique and its different modifications are used as acid fast staining technique. The acid fast staining reaction of Mycobacteria, along with their characteristic size and shape, is a valuable aid in the early detection of infection and monitoring of therapy for Mycobacterial diseases. Pus, tissue, lymph node biopsy, any body fluids (Pleural fluid, Ascitic fluid, Pericardial fluid, Peritoneal fluid, Urine), Endotracheal secretion, Bronchoalveolar lavage, Gastric lavage in paediatric patients should be collected in a sterile, leak-proof, screw capped universal container for the ultimate yield of the result. It has been estimated that, approximately 10,000 acid fast bacilli per ml. of sample are required to be detected by routine microscopy. Patients with extensive disease loaded with large numbers of Mycobacteria provides good correlation between positive smears to positive cultures, on the other hand, minimal or less advanced disease shows the correlation rate between 25-40%. Acid fast stain is also useful in prognosis. With detection of smear positivity, anti-tubercular treatment can be started at the earliest, besides with continued treatment more organisms are killed, so assessing the presence or absence of the organism during treatment can provide an early objective measure of response. The number of organisms fail to decrease after therapy is started, the possibility of drug resistance must be considered and additional culture and drug susceptibility studies should be required.